Journal: Annals of Translational Medicine
Article Title: Effects of gamma irradiation on the measurement of hepatitis B virus DNA in dentin harvested from chronically infected patients
doi: 10.21037/atm.2020.03.04
Figure Lengend Snippet: Key resources in HBV DNA measurement
Article Snippet: After processing, Solution C (400 µL) and Proteinase K (20 mg/mL, 50 µL) were added to the resulting supernatant, and the mixture was incubated under gentle agitation at 56 °C for 3 h ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Reagent or resource Source Identifier Reagents Proteinase K Ambion Cat#AM2546 Phenol: chloroform: isoamyl alcohol =25:24:1, saturated with 10 mM Tris, pH 8.0, 1 mM EDTA Sigma Cat#P3803 Critical commercial assays TBONE EX Kit DNA Chip Research Inc. Cat#TBEW200 QIAamp DNA Investigator Kit Qiagen Cat#56504 Lyophilsed 2X qPCR MasterMix Primerdesign Ltd. Cat#oasig-standard-150 Real-time PCR detection kit for hepatitis B virus MyBiosource Inc. Cat#MBS486092 Software SDS 2.4.1 Applied Biosystems https://www.thermofisher.com/kr/ko/home/technical-resources/software-downloads/applied-biosystems-7900ht-fast-real-timespcr-system.html Open in a separate window caption a8 Key resources in HBV DNA measurement The resultant pure DNA solution from each sample was obtained using an QIAamp DNA Investigator Kit (Qiagen, Hilden, Germany, ).
Techniques: Real-time Polymerase Chain Reaction, Virus, Software